Anti-DRIP130抗体The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA.

产品编号 yb-13032R
英文名称 Anti-DRIP130抗体
中文名称 转录共激活因子130抗体
别    名 130 kDa transcriptional co activator; 133 kDa transcriptional co activator; Activator recruited cofactor 130 kDa component; Activator-recruited cofactor 130 kDa component; ARC 130; ARC130; Cofactor required for Sp1 transcriptional activation subunit 3 130kDa; Cofactor required for Sp1 transcriptional activation subunit 3 130kDa mediator; Cofactor required for Sp1 transcriptional activation subunit 3; CRSP 130; CRSP 130 kD subunit; CRSP 133; CRSP 3; CRSP complex subunit 3; CRSP130; CRSP133; CRSP3; CRSP3 protein; DKFZp434H0117; DRIP 130; DRIP130; hSur-2; KIAA1216; MED 23; MED23; MED23_HUMAN; Mediator complex subunit 23; Mediator of RNA polymerase II transcription subunit 23; Protein sur-2 homolog; SUR 2; SUR2; Transcriptional co activator CRSP 130; Transcriptional co activator CRSP130; Transcriptional coactivator CRSP130; Vitamin D3 receptor interacting protein; Vitamin D3 receptor interacting protein complex 130 kDa component; Vitamin D3 receptor-interacting protein complex 130 kDa component.
 Anti-DRIP130抗体  
说 明 书 0.1ml  0.2ml  
研究领域 细胞生物  染色质和核信号  信号转导  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 156kDa
细胞定位 细胞核 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human DRIP130/CRSP130
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
 
PubMed PubMed
产品介绍 background:
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2012]

Function:
Required for transcriptional activation subsequent to the assembly of the preinitiation complex. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.

Subunit:
Interacts with ELK1 (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with CEBPB, CTNNB1, and GLI3. Interacts with the adenovirus E1A protein.