Anti-CHMP1A抗体Component of the ESCRT-III complex, which is required for multivesicular bodies （MVBs） formation and sorting of endosomal cargo proteins into MVBs.

产品编号 yb-4207R
英文名称 Anti-CHMP1A抗体
中文名称 染色质修饰蛋白1A抗体
别    名 CHMP1A; Charged multivesicular body protein 1a; Chromatin modifying protein 1a; hVps46 1; Metalloprotease 1; PCOLN3; Procollagen (type III) N endopeptidase; PRSM1; Vacuolar protein sorting 46 1; Vps46 1; CHM1A_HUMAN.
Anti-CHMP1A抗体 
说 明 书 0.2ml  
研究领域 肿瘤  细胞生物  免疫学  信号转导  细胞周期蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 22kDa
细胞定位 细胞核 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human CHMP1A
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
Component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein PCGF4/BMI1 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargoes of viral proteins.

Function:
Probable peripherally associated component of theendosomal sorting required for transport complex III (ESCRT-III)which is involved in multivesicular bodies (MVBs) formation andsorting of endosomal cargo proteins into MVBs. MVBs containintraluminal vesicles (ILVs) that are generated by invagination andscission from the limiting membrane of the endosome and mostly aredelivered to lysosomes enabling degradation of membrane proteins,such as stimulated growth factor receptors, lysosomal enzymes andlipids. The MVB pathway appears to require the sequential functionof ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostlydissociate from the invaginating membrane before the ILV isreleased. The ESCRT machinery also functions in topologicallyequivalent membrane fission events, such as the terminal stages ofcytokinesis and the budding of enveloped viruses (HIV-1 and otherlentiviruses). ESCRT-III proteins are believed to mediate thenecessary vesicle extrusion and/or membrane fission activities,possibly in conjunction with the AAA ATPase VPS4. Involved incytokinesis. Involved in recruiting VPS4A and/or VPS4B to themidbody of dividing cells. May also be involved in chromosomecondensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4to regions of condensed chromatin. May play a role in stable cellcycle progression and in PcG gene silencing.

Subunit:
Probable peripherally associated component of theendosomal sorting required for transport complex III (ESCRT-III).ESCRT-III components are thought to multimerize to form a flatlattice on the perimeter membrane of the endosome. Several assemblyforms of ESCRT-III may exist that interact and act sequentally.Self-associates. Interacts with CHMP1B. Interacts with VPS4A.Interacts with VPS4B. Interacts with PHF1. Interacts with IST1.

Subcellular Location:
Cytoplasm. Endosome membrane; Peripheral membrane protein. Nucleus matrix.

Tissue Specificity:
Expressed in placenta, cultured skin fibroblasts and in osteoblast cell line MG63.

Similarity:
Belongs to the SNF7 family.

Database links:
UniProtKB/Swiss-Prot: Q9HD42.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.