Anti-CACYBP抗体周期素结合蛋白（Calcyclin-binding-protein, 又称siah-interaction-protein, CacyBP/SIP）与肿瘤的生长、转移相关，是一个26kDa以钙依赖的方式结合S100家族的蛋白,该蛋白的表达，与细胞周期的变化有关。

产品编号 yb-3819R
英文名称 Anti-CACYBP抗体
中文名称 钙周期素结合蛋白抗体
别    名 CACYBP; Calcyclin binding protein; GIG 5; GIG5; Growth inhibiting gene 5; Growth inhibiting gene 5 protein; hCacyBP; PNAS 107; PNAS107; S100A6 binding protein; S100A6BP; SIP.
Anti-CACYBP抗体    
说 明 书 0.1ml  0.2ml  
研究领域 肿瘤  免疫学  信号转导  转录调节因子  结合蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, .,
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 25kDa
细胞定位 细胞核 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human S100A6BP
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
SIAH Interacting Protein may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. 

Function:
May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).

Subunit:
Homodimer. Interacts with proteins of the S100 family S100A1, S100A6, S100B, S100P and S100A12 at physiological calcium concentrations (By similarity). Component of some large E3 complex at least composed of UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Interacts directly with SIAH1, SIAH2 and SKP1.

Subcellular Location:
Nucleus. Cytoplasm. Note=Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.

Post-translational modifications:
Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

钙周期素结合蛋白(Calcyclin-binding-protein, 又称siah-interaction-protein, CacyBP/SIP)与肿瘤的生长、转移相关，是一个26kDa以钙依赖的方式结合S100家族的蛋白,该蛋白的表达，与细胞周期的变化有关。