Anti-EVA1抗体Thymus development depends on a complex series of interactions between thymocytes and the stromal component

产品编号 yb-11080R
英文名称 Anti-EVA1抗体
中文名称 髓鞘蛋白P0样蛋白2抗体
别    名 Epithelial V like antigen 1; epithelial V-like antigen; EVA; EVA1; MPZL2; MPZL2 myelin protein zero like 2; myelin protein zero-like 2; Myelin protein zero like protein 2; MPZL2_HUMAN.
Anti-EVA1抗体 
说 明 书 0.1ml  0.2ml  
研究领域 神经生物学  细胞粘附分子  细胞骨架  细胞外基质  跨膜蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 22kDa
细胞定位 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human MPZL2/EVA1
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
Thymus development depends on a complex series of interactions between thymocytes and the stromal component of the organ. Epithelial V-like antigen (EVA) is expressed in thymus epithelium and strongly downregulated by thymocyte developmental progression. This gene is expressed in the thymus and in several epithelial structures early in embryogenesis. It is highly homologous to the myelin protein zero and, in thymus-derived epithelial cell lines, is poorly soluble in nonionic detergents, strongly suggesting an association to the cytoskeleton. Its capacity to mediate cell adhesion through a homophilic interaction and its selective regulation by T cell maturation might imply the participation of EVA in the earliest phases of thymus organogenesis. The protein bears a characteristic V-type domain and two potential N-glycosylation sites in the extracellular domain; a putative serine phosphorylation site for casein kinase 2 is also present in the cytoplasmic tail. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008].

Function:
Mediates homophilic cell-cell adhesion. 

Subcellular Location:
Cell Membrane; Single-pass type I membrane protein, cytoskeleton

Tissue Specificity:
Expressed in thymocytes and thymic stromal cells; expression elevated in some T-cell leukemias. 

Similarity:
Belongs to the myelin P0 protein family. 
Contains 1 Ig-like V-type (immunoglobulin-like) domain.