Anti-CNOT4抗体C1QTNF4 is a 329 amino acid secreted protein that contains two C1q domains and may play a role in immune homeostasis and various other

产品编号 yb-20045R
英文名称 Anti-CNOT4抗体

complex known to be involved in transcription as well as mRNA degradation. 
中文名称 细胞表面趋化因子受体4相关蛋白4抗体
别    名 CCR4 NOT transcription complex subunit 4; CCR4-associated factor 4; CCR4-NOT transcription complex subunit 4; CLONE243; CNOT 4; Cnot4; CNOT4_HUMAN; E3 ubiquitin-protein ligase CNOT4; NOT 4; NOT4 (negative regulator of transcription 4 yeast) homolog; NOT4; NOT4H; Potential transcriptional repressor NOT4Hp.
Anti-CNOT4抗体  
说 明 书 0.1ml  0.2ml  
研究领域 细胞生物  通道蛋白  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Horse, Rabbit, 
产品应用 WB=1:100-500 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 64kDa
细胞定位 细胞核 细胞浆 分泌型蛋白 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human CNOT4
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription as well as mRNA degradation. Various subunits within the complex are involved in influencing nuclear hormone receptor activities. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome. CNOT4 (CCR4-NOT transcription complex subunit 4), also known as CCR4-associated factor 4 and E3 ubiquitin-protein ligase CNOT4, is a 575 amino acid protein that is a subunit of the CCR4-NOT complex. CNOT4 contains one C3H1-type zinc finger, one RING-type zinc finger and one RRM (RNA recognition motif) domain. Via its RING domain, CNOT4 binds E2 ubiquitin ligases. CNOT4 functions as a UbcH5B-dependent ubiquitin-protein ligase (E3 ligase). There are eight isoforms of CNOT4 that are expressed as a result of alternative splicing events.

Function:
Has E3 ubiquitin ligase activity. The CCR4-NOT complex functions as general transcription regulation complex.

Subunit:
Subunit of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, C, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. Binds C via its C-terminus. Binds E2 ubiquitin ligases via its RING domain. Interacts (via RING domain) with UBE2D2. 

Subcellular Location:
Cytoplasm. Nucleus.

Post-translational modifications:
Autoubiquitinated.

Similarity:
Contains 1 C3H1-type zinc finger. 
Contains 1 RING-type zinc finger. 
Contains 1 RRM (RNA recognition motif) domain.