Anti-CHD1抗体Chromodomains participate in the recognition of lysine-methylated histone tails and nucleic acids.

产品编号 yb-18890R
英文名称 Anti-CHD1抗体
中文名称 ATP依赖的解旋酶CHD1抗体
别    名 ATP dependent helicase CHD 1; ATP dependent helicase CHD1; ATP-dependent helicase CHD1; CHD 1; CHD-1; CHD1; CHD1_HUMAN; Chromodomain helicase DNA binding protein 1; Chromodomain-helicase-DNA-binding protein 1; DKFZp686E2337; OTTHUMP00000222625.
Anti-CHD1抗体  
说 明 书 0.1ml  0.2ml  
研究领域 细胞生物  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 197kDa
细胞定位 细胞核 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human CHD1
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
Chromodomains participate in the recognition of lysine-methylated histone tails and nucleic acids. The CHD1 protein, named for its chromo-domain, ATPase helicase-like domain and DNA-binding domain, functions as an ATP-utilizing chromatin assembly factor. Unlike HP1 and Polycomb proteins that use single chromodomains to bind to their respective methylated Histone H3 tails, the two chromodomains of CHD1 cooperate to associate with one methylated H3 tail. Unique inserts within chromodomain 1 of CHD1 block the expected site of H3 tail binding seen in HP1 and Polycomb, and instead direct H3 binding to a groove at the interchromodomain junction. The human CHD1 gene maps to 5q21.1, and encodes a 1,709 amino acid deduced protein that shares 95.5% identity with the 1,711 amino acid mouse Chd1 polypeptide.

Function:
ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells.

Subcellular Location:
Nucleus. Cytoplasm. Is released into the cytoplasm when cells enter mitosis and is reincorporated into chromatin during ophase-cytokinesis.

Similarity:
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.