Anti-CCDC139抗体Pseudouridination is the isomerization of uridine to pseudouridine. It is the most common posttranscriptional nucleotide modification found in RNA.

产品编号 yb-7102R
英文名称 Anti-CCDC139抗体
中文名称 卷曲螺旋结构域蛋白139抗体
别    名 CCDC139; Coiled coil domain containing protein 139; DOBI; FLJ32312; MGC126729; Putative tRNA pseudouridine synthase Pus10; tRNA pseudouridine 55 synthase; tRNA uridine isomerase; PUS10_HUMAN.
Anti-CCDC139抗体 
说 明 书 0.1ml  0.2ml  
研究领域 细胞生物  信号转导  细胞凋亡  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 60kDa
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human PUS10/CCDC139
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
Pseudouridination is the isomerization of uridine to pseudouridine. It is the most common posttranscriptional nucleotide modification found in RNA. It is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones which facilitate the correct folding and assembly of tRNAs.

Function:
Pseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death.

Post-translational modifications:
Proteolytically cleaved during TRAIL-induced cell death. Cleaved, in vitro, either by caspase-3 or caspase-8.

Similarity:
Belongs to the pseudouridine synthase Pus10 family. 

Database links:
UniProtKB/Swiss-Prot: Q3MIT2.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.