Anti-CKAP5抗体CKAP5 is a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family.

产品编号 yb-5738R
英文名称 Anti-CKAP5抗体
中文名称 结肠和肝肿瘤高表达蛋白抗体
别    名 Ch TOG protein; chTOG; CHTOG protein; CKAP5_HUMAN; CKAP 5; CKAP5; CKAP5 protein; Colonic and hepatic tumor over expressed; Colonic and hepatic tumor over expressed protein; Colonic and hepatic tumor overexpressed protein; Cytoskeleton associated protein 5; FLJ35359; KIAA0097; TOG; TOGp.
Anti-CKAP5抗体   
说 明 书 0.2ml  
研究领域 肿瘤  细胞生物  免疫学  细胞周期蛋白  细胞分化  细胞类型标志物  细胞骨架  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Horse, Rabbit, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 225kDa
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human CKAP5/ch TOG
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
CKAP5 is a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified.

Function:
Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. 

Subunit:
Interacts with TACC1. Interacts with SLAIN2. 

Subcellular Location:
Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Detected on centrosomes during interphase. Detected on spindle poles and microtubules during mitosis. 

Tissue Specificity:
Overexpressed in hepatomas and colonic tumors. Also expressed in skeletal muscle, brain, heart, placenta, lung, liver, kidney and pancreas. 

Similarity:
Belongs to the TOG/XMAP215 family.
Contains 10 HEAT repeats. 

Database links:
UniProtKB/Swiss-Prot: Q14008.3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.