Anti-Amisyn抗体Amisyn is a mostly cytosolic protein related to Tomosyn which plays an important role in SNARE complex assembly.

产品编号 yb-11039R
英文名称 Anti-Amisyn抗体
中文名称 突触融合结合蛋白6抗体
别    名 Amisyn; HSPC156; STXB6_HUMAN; STXBP6; Syntaxin binding protein 6; Syntaxin-binding protein 6.
Anti-Amisyn抗体  
说 明 书 0.1ml  0.2ml  
研究领域 神经生物学  细胞粘附分子  细胞外基质  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Cow, Rabbit, Sheep, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 24kDa
细胞定位 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human Amisyn
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
Amisyn is a mostly cytosolic protein related to Tomosyn which plays an important role in SNARE complex assembly. Amisyn contains a v-SNARE coiled coil homology domain that binds to Syntaxin 1A and weakly to Syntaxin 4. Three isoforms exist for Amisyn. Isoform 1 is the full length protein, isoform 2 has a different amino acid sequence between residues 204-210 and isoform 3 is missing amino acids 1-102 and contains a different sequence for amino acids 103-150. Amisyn lacks a transmembrane domain and therefore is unable to assemble into a functional, membrane-anchored SNARE complex. This suggests that Amisyn may instead be acting to maintain SNARE conformation and facilitate the binding of VAMP-2. Amisyn can inhibit exocytosis independent of Syntaxin binding.

Function:
Forms non-fusogenic complexes with SNAP25 and STX1A and may thereby modulate the formation of functional SNARE complexes and exocytosis.

Subunit:
Part of a ternary complex containing SNAP25 and STX1A that can be dissociated by NAPA and NSF. Interacts with STX4A. 

Subcellular Location:
Cytoplasm. Membrane.

Tissue Specificity:
Detected at low levels in brain, and at very low levels in heart, adrenal gland, testis, liver and kidney.

Similarity:
Contains 1 v-SNARE coiled-coil homology domain.

Database links:
UniProtKB/Swiss-Prot: Q8NFX7.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.