Anti-Alpha 1 microglobulin抗体This gene encodes a complex glycoprotein secreted inplasma. The precursor is proteolytically processed into distinctfunctioning proteins: alpha-1-microglobulin, which belon

产品编号 yb-6512R
英文名称 Anti-Alpha 1 microglobulin抗体
中文名称 α1微球蛋白抗体
别    名 alpha 1 Microglobulin; Alpha 1 microglobulin/bikunin precursor; Alpha-1 microglycoprotein; AMBP; AMBP_HUMAN; Bikunin; Complex-forming glycoprotein heterogeneous in charge; EDC1; Growth inhibiting protein 19; HCP; HI 30; HI-30; HI30; IATIL; Inter alpha trypsin inhibitor light chain; ITI; ITI LC; ITI-LC; ITIL; ITILC; Kunitz domain; Plasma protein; Protein HC; Proteinase inhibitor; Trypstatin; Uronic-acid-rich protein; UTI
 Anti-Alpha 1 microglobulin抗体  
说 明 书 0.2ml  
研究领域 肿瘤  心血管  肿瘤细胞生物标志物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 7/16kDa
细胞定位 分泌型蛋白 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human Protein AMBP (301-344aa)
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
 
PubMed PubMed
产品介绍 background:
This gene encodes a complex glycoprotein secreted inplasma. The precursor is proteolytically processed into distinctfunctioning proteins: alpha-1-microglobulin, which belongs to thesuperfamily of lipocalin transport proteins and may play a role inthe regulation of inflammatory processes, and bikunin, which is aurinary trypsin inhibitor belonging to the superfamily ofKunitz-type protease inhibitors and plays an important role in manyphysiological and pathological processes. This gene is located onchromosome 9 in a cluster of lipocalin genes.

Function:
Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin,and lysosomal granulocytic elastase. Inhibits calcium oxalatecrystallization.
Trypstatin is a trypsin inhibitor 

Subunit:
I-alpha-I plasma protease inhibitors are assembled fromone or two heavy chains (H1, H2 or H3) and one light chain,bikunin. Inter-alpha-inhibitor (I-alpha-I) is composed of H1, H2and bikunin, inter-alpha-like inhibitor (I-alpha-LI) of H2 andbikunin, and pre-alpha-inhibitor (P-alpha-I) of H3 and bikunin.Alpha-1-microglobulin occurs as a monomer and also in complexeswith IgA and albumin. Alpha-1-microglobulin interacts with FN1.Trypstatin is a monomer and also occurs as a complex with tryptasein mast cells (By similarity). Alpha-1-microglobulin and bikunininteract (via SH3 domain) with HEV ORF3 protein.

Subcellular Location:
Secreted.

Tissue Specificity:
Expressed by the liver and secreted in plasma.Alpha-1-microglobulin occurs in many physiological fluids includingplasma, urine, and cerebrospinal fluid. Inter-alpha-trypsininhibitor is present in plasma and urine.

Post-translational modifications:
The precursor is proteolytically processed into separayfunctioning proteins.
3-hydroxykynurenine, an oxidized tryptophan metabolite thatis common in biological fluids, reacts with Cys-53, Lys-111,Lys-137, and Lys-149 to form heterogeneous polycyclic chromophoresincluding hydroxanthommatin. The reaction by alpha-1-microglobulinis autocatalytic; the human protein forms chromophore even whenexpressed in insect and bacterial cells. The chromophore can reactwith accessible cysteines forming non-reducible thioethercross-links with other molecules of alpha-1-microglobulin or withother proteins such as Ig alpha-1 chain C region 'Cys-352'.
Heavy chains are interlinked with bikunin via a chondroitin4-sulfate bridge to the their C-terminal aspartate (By similarity).
N- and O-glycosylated. N-glycan heterogeneity at Asn-115:Hex5HexNAc4 (major), Hex6HexNAc5 (minor) and dHex1Hex6HexNAc5(minor). N-glycan at Asn-250: Hex5HexNAc4. O-linkage of theglycosaminoglycan, chondroitin sulfate, at Ser-215 allowscross-linking between the three polypeptide chains. 

Similarity:
In the N-terminal section; belongs to the calycinsuperfamily. Lipocalin family. 
Contains 2 BPTI/Kunitz inhibitor domains. 

Database links:

UniProtKB/Swiss-Prot: P02760.1

 
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.