Anti-ATG1/ULK1抗体来自β-淀粉样蛋白原，在脑组织的细胞外呈丝状蛋白样沉积 ，是淀粉样结节性神经炎病变的主要蛋白成分，在神经纤维中也有沉积。在老年性痴呆Alzheimer病中，大脑皮质中特征性地出现β-淀粉样蛋白沉积形成的老年斑。

产品编号 yb-3602R
英文名称 Anti-ATG1/ULK1抗体
中文名称 自噬相关蛋白1抗体
别    名 ATG 1; ULK1; ATG1; ATG1A; Serine/threonine protein kinase ULK1; Serine/threonine protein kinase Unc51.1; ULK 1; Unc 51 (C. elegans) like kinase 1; UNC 51; Unc 51 like kinase 1; Unc-51-like kinase 1; Unc-51 like kinase 1 (C. elegans); UNC51; ULK1_HUMAN; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; hATG1.
nti-ATG1/ULK1抗体    
说 明 书 0.1ml  0.2ml  
研究领域 肿瘤  细胞生物  免疫学  信号转导  细胞凋亡  转录调节因子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:10-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 116kDa
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human ATG1/ULK1
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
nti-ATG1/ULK1抗体
PubMed PubMed
产品介绍 background:
ULK1 belongs to the serine/threonine protein kinase family. It is involved in axon growth and plays an essential role in neurite branching during sensory axon outgrowth. Knockdown of ULK1 results in impaired endocytosis of nerve growth factor (NGF), excessive axon arborization, and severely stunted axon elongation indicating that ULK1 mediates a non clathrin coated endocytosis in sensory growth cones. Knockdown of ULK1 also inhibits the autophagic response. It appears to act as a convergence point for multiple signals that regulate autophagy, and in turn interacts with a large number of autophagy related (Atg) proteins.

Function:
Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.

Subunit:
Interacts with GABARAP and GABARAPL2. Interacts (via C-terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation.

Subcellular Location:
Cytoplasm, cytosol. Preautophagosomal structure. Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.

Tissue Specificity:
Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

Post-translational modifications:
Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. 
Contains 1 protein kinase domain.

nti-ATG1/ULK1抗体
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

Atg1是一种*/*蛋白激酶。Atg1的激脢活性是CVT信号传导通路以及細胞自噬所必須的。Atg1可以和一些执行细胞自噬的蛋白相互作用，而且有很多调控細胞自噬的信号传导通路汇集在Atg1。因此Atg1可能是一个可以调控細胞自噬很多步驟的一个调节关键点。但在较高等的真核生物中，Atg1的角色仍然不是很清楚。通过目前的研究已经比较了解细胞自噬可以导致细胞的死亡，但是如何导致死亡的分子机制还不清楚，有待于进一步研究。